| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Rondônia. |
|
Data corrente: |
08/02/2017 |
|
Data da última atualização: |
10/11/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
CAMPOS, F. T. de; RINCON, J. A. A.; ACOSTA, D. A. V.; SILVEIRA, P. A. S.; PRADIEÉ, J.; CORRÊA, M. N.; GASPERIN, B. G.; PFEIFER, L. F. M.; BARROS, C. C.; PEGORARO, L. M. C.; SCHNEIDER, A. |
|
Afiliação: |
Felipe Terres de Campos, UFPel; Joao Aveiro Alvarado Rincon, UFPel; Diego Andres Velasco Acosta, CORPOICA; Pedro Augusto Silva Silveira, UFPel; Jorgea Pradieé, Embrapa Clima Temperado; Marcio Nunes Corrêa, UFPel; Bernardo Garziera Gasperin, UFPel; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-Rondonia; Carlos Castilho Barros, UFPel; LIGIA MARGARETH CANTARELLI PEGORARO, CPACT; Augusto Schneider, UFPel. |
|
Título: |
The acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Theriogenology, v. 89, p. 244 - 249, 2017. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells. MenosThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic a... Mostrar Tudo |
|
Palavras-Chave: |
Esteroidogênese; PON1. |
|
Thesagro: |
Colesterol; Inflamação. |
|
Thesaurus Nal: |
cholesterol; inflammation; steroidogenesis. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/227597/1/cpafro-17770.pdf
|
|
Marc: |
LEADER 02572naa a2200325 a 4500
001 2063230
005 2021-11-10
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, F. T. de
245 $aThe acute effect of intravenous lipopolysaccharide injection on serumand intrafollicular HDL components and gene expression in granulosacells of the bovine dominant follicle.$h[electronic resource]
260 $c2017
520 $aThe aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) ? estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
650 $acholesterol
650 $ainflammation
650 $asteroidogenesis
650 $aColesterol
650 $aInflamação
653 $aEsteroidogênese
653 $aPON1
700 1 $aRINCON, J. A. A.
700 1 $aACOSTA, D. A. V.
700 1 $aSILVEIRA, P. A. S.
700 1 $aPRADIEÉ, J.
700 1 $aCORRÊA, M. N.
700 1 $aGASPERIN, B. G.
700 1 $aPFEIFER, L. F. M.
700 1 $aBARROS, C. C.
700 1 $aPEGORARO, L. M. C.
700 1 $aSCHNEIDER, A.
773 $tTheriogenology$gv. 89, p. 244 - 249, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Rondônia (CPAF-RO) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Agricultura Digital. Para informações adicionais entre em contato com cnptia.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Agricultura Digital. |
|
Data corrente: |
22/06/2007 |
|
Data da última atualização: |
06/12/2007 |
|
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
|
Circulação/Nível: |
-- - -- |
|
Autoria: |
ALMEIDA, G. M. de B.; ALUÍSIO, S. M.; OLIVEIRA, L. H. M. de. |
|
Afiliação: |
Gladis Maria de Barcellos, UFSCAR; Sandra Maria Aluísio, USP-ICMC; Leandro Henrique Mendonça de Oliveira, CNPTIA. |
|
Título: |
O método em terminologia: revendo alguns procedimentos. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
In: ISQUERDO, A. N.; ALVES, I. M. (Org.). As ciências do léxico: lexicologia: lexicografia: terminologia. Campo Grande, MS: Ed. UFMS; São Paulo: Humanitas, 2007. |
|
Páginas: |
p. 409-420. |
|
Idioma: |
Português |
|
Palavras-Chave: |
Ontologia. |
|
Thesagro: |
Terminologia. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 00587naa a2200169 a 4500
001 1004372
005 2007-12-06
008 2007 bl uuuu u00u1 u #d
100 1 $aALMEIDA, G. M. de B.
245 $aO método em terminologia$brevendo alguns procedimentos.
260 $c2007
300 $ap. 409-420.
650 $aTerminologia
653 $aOntologia
700 1 $aALUÍSIO, S. M.
700 1 $aOLIVEIRA, L. H. M. de
773 $tIn: ISQUERDO, A. N.; ALVES, I. M. (Org.). As ciências do léxico: lexicologia: lexicografia: terminologia. Campo Grande, MS: Ed. UFMS; São Paulo: Humanitas, 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Expressão de busca inválida. Verifique!!! |
|
|
